Characterization of Phn15.1, a Newly Identified Phytophthora nicotianae Resistance QTL in Nicotiana tabacum.
Identifieur interne : 000277 ( Main/Exploration ); précédent : 000276; suivant : 000278Characterization of Phn15.1, a Newly Identified Phytophthora nicotianae Resistance QTL in Nicotiana tabacum.
Auteurs : Justin M. Ma [États-Unis] ; Crystal B. Heim [États-Unis] ; Matt Humphry [Royaume-Uni] ; Jessica M. Nifong [États-Unis] ; Ramsey S. Lewis [États-Unis]Source :
- Plant disease [ 0191-2917 ] ; 2020.
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Abstract
Phytophthora nicotianae is an oomycete that causes black shank, one of the most economically important diseases affecting tobacco production worldwide. Identification and introgression of novel genetic variability affecting partial genetic resistance to this pathogen is important because of the increased durability of partial resistance over time as compared with genes conferring immunity. A previous mapping study identified a quantitative trait locus (QTL), hereafter designated as Phn15.1, with a major effect on P. nicotianae resistance in tobacco. In this research, we describe significantly improved resistance of nearly isogenic lines (NILs) of flue-cured tobacco carrying the introgressed Phn15.1 region derived from highly resistant cigar tobacco cultivar Beinhart 1000. The Phn15.1 region appeared to act in an additive or partially dominant manner to positively affect resistance. To more finely resolve the position of the gene or genes underlying the Phn15.1 effect, the QTL was mapped with an increased number of molecular markers (single-nucleotide polymorphisms) identified to reside within the region. Development and evaluation of subNILs containing varying amounts of Beinhart 1000-derived Phn15.1-associated genetic material permitted the localization of the QTL to a genetic interval of approximately 2.7 centimorgans. Importantly, we were able to disassociate the Beinhart 1000 Phn15.1 resistance alleles from a functional NtCPS2 allele(s) which contributes to the accumulation of a diterpene leaf surface exudate considered undesirable for flue-cured and burley tobacco. Information from this research should be of value for marker-assisted introgression of Beinhart 1000-derived partial black shank resistance into flue-cured and burley tobacco breeding programs.
DOI: 10.1094/PDIS-10-19-2257-RE
PubMed: 32310718
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Ma</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Heim, Crystal B" sort="Heim, Crystal B" uniqKey="Heim C" first="Crystal B" last="Heim">Crystal B. Heim</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Humphry, Matt" sort="Humphry, Matt" uniqKey="Humphry M" first="Matt" last="Humphry">Matt Humphry</name><affiliation wicri:level="1"><nlm:affiliation>Plant Biotechnology Division, British American Tobacco Company, Cambridge, U.K.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Plant Biotechnology Division, British American Tobacco Company, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Nifong, Jessica M" sort="Nifong, Jessica M" uniqKey="Nifong J" first="Jessica M" last="Nifong">Jessica M. Nifong</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Lewis, Ramsey S" sort="Lewis, Ramsey S" uniqKey="Lewis R" first="Ramsey S" last="Lewis">Ramsey S. Lewis</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles (MeSH)</term><term>Phytophthora (MeSH)</term><term>Plant Diseases (MeSH)</term><term>Quantitative Trait Loci (MeSH)</term><term>Tobacco (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allèles (MeSH)</term><term>Locus de caractère quantitatif (MeSH)</term><term>Maladies des plantes (MeSH)</term><term>Phytophthora (MeSH)</term><term>Tabac (MeSH)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Phytophthora</term><term>Plant Diseases</term><term>Quantitative Trait Loci</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allèles</term><term>Locus de caractère quantitatif</term><term>Maladies des plantes</term><term>Phytophthora</term><term>Tabac</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Phytophthora nicotianae</i> is an oomycete that causes black shank, one of the most economically important diseases affecting tobacco production worldwide. Identification and introgression of novel genetic variability affecting partial genetic resistance to this pathogen is important because of the increased durability of partial resistance over time as compared with genes conferring immunity. A previous mapping study identified a quantitative trait locus (QTL), hereafter designated as <i>Phn15.1,</i> with a major effect on <i>P. nicotianae</i> resistance in tobacco. In this research, we describe significantly improved resistance of nearly isogenic lines (NILs) of flue-cured tobacco carrying the introgressed <i>Phn15.1</i> region derived from highly resistant cigar tobacco cultivar Beinhart 1000. The <i>Phn15.1</i> region appeared to act in an additive or partially dominant manner to positively affect resistance. To more finely resolve the position of the gene or genes underlying the <i>Phn15.1</i> effect, the QTL was mapped with an increased number of molecular markers (single-nucleotide polymorphisms) identified to reside within the region. Development and evaluation of subNILs containing varying amounts of Beinhart 1000-derived <i>Phn15.1</i>-associated genetic material permitted the localization of the QTL to a genetic interval of approximately 2.7 centimorgans. Importantly, we were able to disassociate the Beinhart 1000 <i>Phn15.1</i> resistance alleles from a functional <i>NtCPS2</i> allele(s) which contributes to the accumulation of a diterpene leaf surface exudate considered undesirable for flue-cured and burley tobacco. Information from this research should be of value for marker-assisted introgression of Beinhart 1000-derived partial black shank resistance into flue-cured and burley tobacco breeding programs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32310718</PMID><DateCompleted><Year>2020</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>06</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>104</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Characterization of <i>Phn15.1</i>, a Newly Identified <i>Phytophthora nicotianae</i> Resistance QTL in <i>Nicotiana tabacum</i>.</ArticleTitle><Pagination><MedlinePgn>1638-1646</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-10-19-2257-RE</ELocationID><Abstract><AbstractText><i>Phytophthora nicotianae</i> is an oomycete that causes black shank, one of the most economically important diseases affecting tobacco production worldwide. Identification and introgression of novel genetic variability affecting partial genetic resistance to this pathogen is important because of the increased durability of partial resistance over time as compared with genes conferring immunity. A previous mapping study identified a quantitative trait locus (QTL), hereafter designated as <i>Phn15.1,</i> with a major effect on <i>P. nicotianae</i> resistance in tobacco. In this research, we describe significantly improved resistance of nearly isogenic lines (NILs) of flue-cured tobacco carrying the introgressed <i>Phn15.1</i> region derived from highly resistant cigar tobacco cultivar Beinhart 1000. The <i>Phn15.1</i> region appeared to act in an additive or partially dominant manner to positively affect resistance. To more finely resolve the position of the gene or genes underlying the <i>Phn15.1</i> effect, the QTL was mapped with an increased number of molecular markers (single-nucleotide polymorphisms) identified to reside within the region. Development and evaluation of subNILs containing varying amounts of Beinhart 1000-derived <i>Phn15.1</i>-associated genetic material permitted the localization of the QTL to a genetic interval of approximately 2.7 centimorgans. Importantly, we were able to disassociate the Beinhart 1000 <i>Phn15.1</i> resistance alleles from a functional <i>NtCPS2</i> allele(s) which contributes to the accumulation of a diterpene leaf surface exudate considered undesirable for flue-cured and burley tobacco. Information from this research should be of value for marker-assisted introgression of Beinhart 1000-derived partial black shank resistance into flue-cured and burley tobacco breeding programs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Justin M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Heim</LastName><ForeName>Crystal B</ForeName><Initials>CB</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Humphry</LastName><ForeName>Matt</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Plant Biotechnology Division, British American Tobacco Company, Cambridge, U.K.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nifong</LastName><ForeName>Jessica M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lewis</LastName><ForeName>Ramsey S</ForeName><Initials>RS</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1583-5978</Identifier><AffiliationInfo><Affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, U.S.A.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000483" MajorTopicYN="N">Alleles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="Y">Phytophthora</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040641" MajorTopicYN="N">Quantitative Trait Loci</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Nicotiana</Keyword><Keyword MajorTopicYN="N">Phytophthora</Keyword><Keyword MajorTopicYN="N">black shank</Keyword><Keyword MajorTopicYN="N">partial resistance</Keyword><Keyword MajorTopicYN="N">plant disease resistance</Keyword><Keyword MajorTopicYN="N">quantitative trait locus</Keyword><Keyword MajorTopicYN="N">tobacco</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32310718</ArticleId><ArticleId IdType="doi">10.1094/PDIS-10-19-2257-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Ma, Justin M" sort="Ma, Justin M" uniqKey="Ma J" first="Justin M" last="Ma">Justin M. Ma</name></region><name sortKey="Heim, Crystal B" sort="Heim, Crystal B" uniqKey="Heim C" first="Crystal B" last="Heim">Crystal B. Heim</name><name sortKey="Lewis, Ramsey S" sort="Lewis, Ramsey S" uniqKey="Lewis R" first="Ramsey S" last="Lewis">Ramsey S. Lewis</name><name sortKey="Nifong, Jessica M" sort="Nifong, Jessica M" uniqKey="Nifong J" first="Jessica M" last="Nifong">Jessica M. Nifong</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Humphry, Matt" sort="Humphry, Matt" uniqKey="Humphry M" first="Matt" last="Humphry">Matt Humphry</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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